Regulatory

Part:BBa_K3544011:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


pTEF1: a stable constitutive promoter in yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168


Design Notes

pTEF1 is a constitutive promoter in plasmid Y26TEF-GDP. We used this promoter for the construction of many plasmid. For more imformation, please view https://2020.igem.org/Team:SCU-China/Design.


Source

S. cerevisiae

References

Partow, Siavash et al. “Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae.” Yeast (Chichester, England) vol. 27,11 (2010): 955-64. doi:10.1002/yea.1806